Stereoselective Separation of Triazole Fungicides Using the ACQUITY UPC2 System and ACQUITY UPC2 Trefoil Chiral Columns

■ ■ Reliable and reproducible measurement of the enantiomeric and/or diastereomeric ratios. IN T RO DU C T IO N The development of analytical methods for the separation of chiral compounds is important in many areas of research, as it is well known that different enantiomers are selectively biologically active. Biochemical reactions can be diastereo or enantioselective. While one isomer may deliver the desired effect to the target species, the other enantiomer may be less effective to the target, completely ineffective, or cause undesirable effects. Additionally, it is known that different isomers can have very different environmental fates. It is estimated that 20 to 30% of pesticides on the market today have optical isomers, and there are reports that 40% of the pesticides used in China are chiral. The study of enantioselectivity is important to the crop protection industry, since the knowledge of the efficacy of each individual enantiomer could facilitate a significant reduction in the total amount of pesticide applied.